香魚片引起食物中毒之首例鑑定報告   全文下載

First Report on Identification of Food Poisoning from Ingestion of Dried Seasoned Puffer Fish Fillet

1998年2月，台中市發生一件疑似食物中毒事件，患者食用香魚片，在食用一口後，即出現口、舌麻痺等類似河魨毒中毒之症狀，其後餵食家中貓、狗，狗食入將近3／4片剩餘之魚片和另外同包三片香魚片，導致狗死亡，貓則僅食入剩餘之香魚片部份產生嘔吐症狀。因此將患者食用同包中剩餘之一片香魚片依河魨毒之生物檢定法測其毒性，得知香魚片每公克具有525老鼠單位之河魨毒性。進一步以的醋酸甲醇溶液抽毒後，經二氯甲烷脫脂、Diaflo YM-1膜超過濾和Bio-Gel P-2管柱層析法精製，部分精製之毒素則以矽土膠體60 F254薄板之薄層色層分析、醋酸纖維片之電泳分析和AM-314分析管柱之逆相高效能液態層析儀分析，得知此香魚片中之毒素含有河魨毒和脫水河魨毒。綜合上述結果，得知本次疑似食物中毒事件之原因物質乃為河魨毒所致。 Dried seasoned puffer fish fillet was suspected for a paralytic food poisoning incident occurred in Taichung, Taiwan, in February 1998. Symptoms included severe numbness of lips. The uneaten portion was analyzed for toxicity and toxic composition. The dried seasoned puffer fish fillet showed a high toxicity of 525 mouse units per gram by tetrodotoxin bioassay. The sample was purified with Diaflo YM-1 membrane ultrafiltration and run through Bio-Gel P-2 chromatography. The purified toxin, analyzed with thin layer chromatography, electrophoresis and high performance liquid chromatography, indicated that the causative toxic agents were tetrodotoxin and anhydrotetrodotoxin."

Dried seasoned puffer fish fillet was suspected for a paralytic food poisoning incident occurred in Taichung, Taiwan, in February 1998. Symptoms included severe numbness of lips. The uneaten portion was analyzed for toxicity and toxic composition. The dried seasoned puffer fish fillet showed a high toxicity of 525 mouse units per gram by tetrodotoxin bioassay. The sample was purified with Diaflo YM-1 membrane ultrafiltration and run through Bio-Gel P-2 chromatography. The purified toxin, analyzed with thin layer chromatography, electrophoresis and high performance liquid chromatography, indicated that the causative toxic agents were tetrodotoxin and anhydrotetrodotoxin.