核酸增幅反應在BCG疫苗與M. smegmatis之鑑別   全文下載

Rapid Differentiation of BCG Vaccine from M. smegmatis by Genomic Amplification

本研究利用的S-23S rRNA spacer基因段鑑別BCG疫苗株，包括Brazilian, Connaught, Danish, Glaxo Japanese, Pasteur, Russian, Taiwan和Tice等9株及另一非疫苗株Mycobacterium smegamatis。使用BCGF1 和BCG R1 作為引子對。結果發現經增幅之PCR產物在疫苗株產生350 bp，對於非疫苗株則產生約456 bp。由本研究所設計之引子對，具快達簡便及有效偵測病原菌之特性並可用於疫苗株菌分離之鑑定，將可嘗試發展為基因探針作為鑑定之工具。"

Amplification of the region of 16S-23S rRNA spacer was performed on 9 isolates of Mycobacterium bovis BCG (including Brazilian, Connaught, Danish, Glaxo, Japanese, Pasteur, Russian, Taiwan and Tice) and one isolate of Mycobacterium smegmatis using BCGFl and BCGRl as the primer pair. In this study we found 350-bp of PCR product in all the BCG strains but a 456-bp amplified fragment from M. smegmatis. Consequently, this primer pair may be a potential tool in differentiating between these two Mycobacterium species. 本研究利用的S-23S rRNA spacer基因段鑑別BCG疫苗株，包括Brazilian, Connaught, Danish, Glaxo Japanese, Pasteur, Russian, Taiwan和Tice等9株及另一非疫苗株Mycobacterium smegamatis。使用BCGF1 和BCG R1 作為引子對。結果發現經增幅之PCR產物在疫苗株產生350 bp，對於非疫苗株則產生約456 bp。由本研究所設計之引子對，具快達簡便及有效偵測病原菌之特性並可用於疫苗株菌分離之鑑定，將可嘗試發展為基因探針作為鑑定之工具。