禽畜產品中拉薩羅（Lasalocid）檢驗方法之探討   全文下載

An Analytical Method for Lasalocid Residues in Livestock Products

利用高效液相層析法建立為雞肉、雞肝、牛肉及牛肝中拉薩羅（lasalocid）殘留量之分析方法。拉薩羅以乙?自檢體中萃取，再加入正己烷去雜質及濃縮後，經矽膠層析匣（silica Sep-Pak cartridge）淨化，最後利用高效液相層析儀以螢光檢測分析。層析管為Cosmosil 5C18-AR-II（5μm，4.6 × 150 mm），移動相為甲醇／0.02M KH2PO4 （84／16），於激發波長310 nm及放射波長420 nm偵測。檢體中添加拉薩羅0.05、0.1及0.2 ppm時，其回收率分別為雞肉93.17～95.61%，雞肝91.53～93.59%，牛肉96.93～97.70%及牛肝90.42～95.92%，變異係數皆小於5%。本方法在四種檢體中之最低檢出限量均為0.005 ppm。利用此方法分析市售雞肉、雞肝、牛肉及牛肝檢體共20件，結果均未檢出。目前我國規定肉品中不得殘留拉薩羅。

A reverse phase high performance liquid chromatographic (RP-HPLC) method for the determination of lasalocid in the muscle and liver of chicken and cattle was developed. Lasalocid was extracted from samples with acetonitrile. After filtration, the extract was purified by liquid-liquid partition, concentrated by rotary evaporation, purified with a silica Sep-Pak cartridge, and finally determined by fluorimetric HPLC. The separation was performed on a Cosmosil 5C18-AR-II column (5 µm, 4.6 x 150 mm) with methanol/0.02M KH2PO4 (84/16, v/v) as the mobile phase, and lasalocid was detected at Ex 310 nm and Em 420 nm. Recovery studies were performed at the 0.05, 0.1 and 0.2 ppm fortification levels in each of the four commodities. Average recoveries were in the range of 93.17~95.61, 91.53~93.59, 96.93~97.70 and 90.42~95.92% from chicken muscle and liver, and bovine muscle and liver, respectively. Coefficients of variation for average recoveries were less than 5%. The limit of detection was 0.005 ppm in these four commodities. When 20 samples purchased from various markets in Taipei were analyzed, no lasalocid was detected. 利用高效液相層析法建立為雞肉、雞肝、牛肉及牛肝中拉薩羅（lasalocid）殘留量之分析方法。拉薩羅以乙腈自檢體中萃取，再加入正己烷去雜質及濃縮後，經矽膠層析匣（silica Sep-Pak cartridge）淨化，最後利用高效液相層析儀以螢光檢測分析。層析管為Cosmosil 5C18-AR-II（5μm，4.6 × 150 mm），移動相為甲醇／0.02M KH2PO4 （84／16），於激發波長310 nm及放射波長420 nm偵測。檢體中添加拉薩羅0.05、0.1及0.2 ppm時，其回收率分別為雞肉93.17～95.61%，雞肝91.53～93.59%，牛肉96.93～97.70%及牛肝90.42～95.92%，變異係數皆小於5%。本方法在四種檢體中之最低檢出限量均為0.005 ppm。利用此方法分析市售雞肉、雞肝、牛肉及牛肝檢體共20件，結果均未檢出。目前我國規定肉品中不得殘留拉薩羅。