傳統中藥成分的液相色譜／質譜／質譜之分析   全文下載

Characterization of Traditional Chinese Medicine by Liquid Chromatography/Atmospheric Pressure Ionization Mass Spectrometry

瀉心湯（Xiexin decoction）用於治療感染性疾病，上消化道出血等疾病已逾百年，但其療效機理尚未明瞭。本文件者採用配有離子噴霧源的液相色譜／質譜／質譜儀（HPLC／MS／MS）對瀉心湯的萃取物進行了定性和定量的分析，用實例說明了如何使用HPLC／MS／MS以對此類混合物進行分析。實驗結果表明，從瀉心湯萃取物中所得到的幾個活性成分的質譜圖與其標準樣品取得了一致的結果，而多個活性成分的濃度，通過一次HPLC進樣即可精確測得。

The decoction of the Xiexin (Shan - Huang- Xiexin - Tang, originated from jin kui Yoa Lue Fang Lun by Zhang Zhongjing) has been in use for centuries to treat illnesses such as: infectious disease, hemorrhage of the upper digestive tract, angiocardiopathy, cerebrovascular disease and immune disease. Although, the decoction of the Xiexin is effective in treating these diseases, its remedial mechanism is not well understood. In this study we examined extracts from the decoction of Xiexin by using HPLC/MS/MS equipped with a ThrbolonSpray source. The simultaneous determination of mixtures of active ingredients in these extracts by HPLC/MS/MS was demonstrated. Product ion spectra of several components in the extracts was obtained and matched against known standards. Concentrations of these active ingredients were estimated by multiple reaction monitoring in the sequential positive and negative ion modes using a single HPLC run. 瀉心湯（Xiexin decoction）用於治療感染性疾病，上消化道出血等疾病已逾百年，但其療效機理尚未明瞭。本文件者採用配有離子噴霧源的液相色譜／質譜／質譜儀（HPLC／MS／MS）對瀉心湯的萃取物進行了定性和定量的分析，用實例說明了如何使用HPLC／MS／MS以對此類混合物進行分析。實驗結果表明，從瀉心湯萃取物中所得到的幾個活性成分的質譜圖與其標準樣品取得了一致的結果，而多個活性成分的濃度，通過一次HPLC進樣即可精確測得。