利用高效液相層析法分析定量Quinolone類抗菌劑   全文下載

Simultaneous Determination of Six Quinolone Antibacterial Agents by HPLC

本文提供一簡單、快速之逆相高效液相層析法，可同時鑑別六種quinolone類藥物，cinoxacin，nalidixic acid，pipemidic acid，ofloxacin，pefloxacin及ciprofloxacin。本法採用之分析管柱為Cosmosil 5C18-MS column （25cm × 4.6 mm），移動相為3mM sodium lauryl sulfate ／CH3OH ： 40 mM phosphate buffer ： CH3CN （25：55：20）混液，以磷酸調整pH值至3.50，移動相之流速為0.8 mL／min，以波長257nm之紫外光偵測。本法以piroxicam為內部標準品可以於40分鐘內完全分離上述之六種quinolone類藥物，經確效結果，分離度separation factor（α）為1.14～2.89，cinoxacin濃度於1μg／mL～50μg／mL間，其他五種成分濃度於5μg／mL～100μg／mL間之線性關係良好，其精密度及準確度頗佳，應用此方法亦可檢測市售檢體，可快速分析定量該類藥物及製劑。

A simple, rapid and reliable high performance liqnid chromatographic method for simultaneous determination of six quinolones (cinoxacin, nalidixic acid, pipemidic acid, ofloxacin, pefloxacin and ciprofloxacin) has been developed. Isocratic separation was achieved using a Nacalai Cosmosil 5C18-MS column (25cm x 4.6 mm J.D.) with a mobile phase consisting of 3mM sodium Iauryl sulfate in methanol : 40mM phosphate buffer : acetonitrile (25:55:20) pH=3.50. An ultraviolet absorption photometer was used as the detector at the wavelength of 257nm. The HPLC separation was completed in 40 min, giving an excellent resolution and peak shape. Piroxicam was used as the internal standard for the assay. The precision, accuracy and reproducibility were satisfactory. The system can be used for assay of six quinolones in dosage forms as well as for identification of these six quinolones in unknown samples.