中藥摻加Sulfamethoxazole之定量探討   全文下載

Determination of Sulfamethoxazole Adulterated in Traditional Chinese Medicine

本研究探討某中醫診所調劑作為治療性病?疹之早、中及晚三餐餐後服用之三種藥粉中所摻加之西藥及其含量。該三種檢體數量分別為11、11及12包，其各包間之重量差異頗大，其最小包至最大包之重量及相對標準偏差依序為，2.844-4.420g、13.2%；2.808-3.782g、9.2%及2.654-3.723g、9.2%。經確認試驗結果，早及中餐後服用之檢體檢出含sulfamethoxazole西藥成分，繼以高效液相層析儀分析sulfamethoxazole之含量，採用C18逆相層析管柱，以乙?：水（含1%醋酸） = 18：82作沖提液分析。sulfamethoxazole之回歸方程式為Y = 240.78X－3.50（r=0.9999），顯示良好之線性關係；同日間及異日間之相對標準偏差分別為1.2%及0.2% （n=5），顯示再現性佳；添加回收率在93.9-97.0%之間，顯示準確性佳，可用於中藥品摻加sulfamethoxazole西藥成分之分析。早及中餐飯後服用之兩種檢體中sulfamethoxazole之平均含量分別為395 mg及345 mg。

In this study, three kinds of traditional Chinese medicine obtained from a traditional Chinese physician's clinic for curing herpes were analyzed. Each kind of medicine was dispensed for use after breakfast, lunch and dinner. For each meal, II, II and 12 doses were collected, respectively. The weight distribution ranges and their coefficient of variation among doses of each kind medicine were 2.822-4.420g, 13.2%, 2.808-3. 782g, 9.2% and 2.654-3. 723g, 9.2%, respectively. Sulfamethoxazole was detected in the doses dispensed for breakfast and lunch but not found in the dose for dinner. Sulfamethoxazole in the samples were assayed by high performance liquid chromatography on C 18 column. The linear calibration range was 0.1-0.8 mg/ml (r=0.9999) and recovery was 93.9-97.0%. The relative standard deviations for intraday and interday analysis were 1.2% and 0.2% (n=5), respectively. The average content of sulfamethoxazole in the dose for breakfast and lunch were 395 mg and 345 mg, respectively.