雞肉及雞肝中衣索巴檢驗方法之探討   全文下載

Studies on the Analysis of Ethopabate in Chicken Meat and Livers

利用高效液相層析法分析雞肉及雞肝中衣索巴之檢驗方法業已建立。衣索巴以乙?自檢體中萃取，經過濾，以乙?飽和之正己烷除去雜質後濃縮，再以矽酸鎂管柱淨化，最後利用高效液相層析儀分析定量。使用之層析管柱為Lichrospher 100 RP-18（25 cm × 4.6 mm i. d.，粒子大小為5μm），移動相為乙?／水（3／7），以螢光輸出器於激發波長300nm及放射波長350nm檢測。於檢體中添加衣索巴0.02，0.05，0.10及0.20ppm時，其回收率於雞肉及雞肝中分別為93.22～94.67%及92.77～94.45%，變異係數皆小於4%，本方法在此二種檢體中之最低檢出量可低達2 ppb。將此方法應用於市售雞肉及雞肝之檢驗，檢體各為25件，結果均未檢出。

A high performance liquid chromatographic (HPLC)method for the determination of ethopabate in chicken meat and liver was developed. Ethopabate was extracted from samples with acetonitrile, and filtered. The extract was partitioned with n-hexane, concentrated to dryness, cleaned up through Florisil column, and quantitatively analyzed by HPLC. A Lichrospher 100 PR-18 (25crn × 4. 6 mm i. d., packed with 5µm particle size) and a solvent system of acetonitrile/water (3/7)with a flow rate of 1.0 ml/min were used. Ethopabates were detected by fluorescence with excitation and emission wavelengths at 300 nm and 350 nm, respectively. The average recoveries of ethopabate spiked into samples at the levels of 0.02, 0.05, 0.10 and 0. 20 ppm were in range of 93.22～94.67% from meat and 92.77～94.45% from liver. Coefficients of variation were less than 4%. The detection limit was 2 ppb. Twenty-five samples each of chicken meat and liver purchased from various markets in Taipei were investigated. Results showed that no ethopabate was detected.