探討不同粒徑SiO2-Allicin抗菌微粒之吸附量及抑菌效果

Effect of particle sizes on the absorption capacity and antimicrobial activity of the SiO2-allicin particles

本研究的目的在探討不同粒徑（<25μm、25~44μm 和44~74μm）之SiO2-Allicin 抗菌微粒對allicin 的吸附能力，對金黃色葡萄球菌、大腸桿菌的抑菌能力及不同重量（0.02、0.05、0.08 和0.1g）下SiO2-Allicin 抗菌微粒對黴菌的抑菌能力，找出最佳抗菌能力的SiO2-Allicin抗菌微粒，以應用在食品包裝上。實驗方法主要利用分光光度計測定不同粒徑SiO2-Allicin抗菌微粒中allicin 的吸附量。利用SiO2-Allicin 抗菌微粒在培養皿中揮發allicin 形成抑菌圈，再由抑菌圈大小換算抑菌率的方式，來探討SiO2-Allicin 抗菌微粒對金黃色葡萄球菌、大腸桿菌及黴菌的抑制能力。實驗結果得到<25μm 粒徑的SiO2-Allicin 抗菌微粒對allicin的吸附量為11.09±0.41mg/g，與44~74μm 粒徑的抗菌粒子（9.85±0.33 mg/g）及25~44μm 粒徑的抗菌粒子（10.18±0.48mg/g） 之間有顯著差異（p<0.05）。探討微生物的抑菌能力方面，從大腸桿菌與金黃色葡萄球菌抑菌率來看，大腸桿菌抑菌率為58.91±0.67%，金黃色葡萄球菌為75.58±1.16%，兩者之間有顯著性差異（p<0.05），也就是說allicin 對於大腸桿菌的抑制能力小於金黃色葡萄球菌。探討抗菌粒子的量對黴菌的抑制能力來看，SiO2-Allicin抗菌微粒的量越多，其抑菌能力越強，隨著儲存天數（1~5 天）的增加，不同重量（0.02~0.08g）抗菌粒子的抑菌能力逐漸降低，跟對照組比較含有0.1g 抗菌粒子的抑菌率，可以維持五天仍為100%。

This study investigated the effect of different particle sizes on the absorption capacity and theantibacterial activity, and the effect of different particle weights on the antifungal activity of theSiO2-allicin particles. Three sizes (<25 μm, 25-44 μm, and 44-74 μm) of the particles were usedto determine the absorption capacity of allicin and antibacterial activity against Staphylococcusaureus and Eschericia coli. Four weights (0.02, 0.05, 0.08, and 0.1 g) of the particles were usedto determine the antifungal activity against Aspergillus nige. Results showed that the absorptioncapacity of allicin obtained in the particle sizes of <25 μm, 25-44 μm, and 44-74 μm were11.09±0.41 mg/g, 10.18±0.48 mg/g, and 9.85±0.33 mg/g, respectively; there was statisticallysignificant difference (p<0.05). It indicates that the smallest particles had a best absorptioncapacity. The antibacterial activity of the SiO2-allicin particles with the size of <25 μm showed thatthe inhibition rate on Staphylococcus aureus and Eschericia coli were 75.58±1.16% and58.91±0.67%, respectively; there was a significant difference (p<0.05). It indicates that theSiO2-allicin particles with the size of <25 μm had a better antibacterial capacity on Staphylococcusaureus than on Eschericia coli. The effect of particle weights on the antifungal activity against Aspergillus niger showed that the more the SiO2-allicin particles added (0.02-0.08 g), the strongerthe antifungal capacity obtained during a 5-day storage. In addition, the antifungal capacity of the0.02-0.08 g particles decreased as the storage time increased. Compared to the control, theantifungal capacity of the 0.1 g SiO2-allicin particles remained a 100% inhibition rate up to Day 5.