| 英文摘要 |
Objectives. This study examined the effect of delayed-entry of blood culture bottlesloaded toBacT/ALERT 3D automated system on the time to detection and positive rate fordetection ATCC strains.Methods. The blood culture bottles were inoculated with whole blood containing microorganisms, and then pre-incubated at various temperatures (4°C, 22°C, 28°C, 35°C and 42°C) for 4hr, 8hr, 12hr, 24hr, and 48 hr prior to loading into an automated system. Results. Mean TTD was inversely correlated with the pre-incubation temperature for most microorganisms (p<0.05), except for H. influenza or N. gonorrhoeae. Organisms in bottles inoculated with the A. baumannii, P. aeruginosa and B.thetaiotaomicron remained undetected by the BacT/Alert system when set at 35°C/48hr, 22°C/48hr, 28°C/24 and 48hr, 35°C/24 and 48hr and 42°C/24 and 48hr, or 42°C/48hr, respectively. However, when all bottles were subjected to subculture for recovery the bacteria, they were found to produce false negative findings in bacterial growth of A. baumannii, P. aeruginosa and B.thetaiotaomicron. Both the positivity rate of detection and culture positivity were decreased significantly for C. albicans, N. gonorrhoeae and S. pyogenes under the condition of delayed entry. E. faecalis and H. influenza were pre-incubated at 4°C/48 hr or 42°C/48 hr, respectively. Conclusion. Taken together, these results suggest that all bottles from clinical settings should be maintained at 22°C or 28°C(RT) for up to24 hr before loading. (J Med Health. 2016;5(1):53-66) |
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