併用半透模透析，肉羹增菌及實驗動物毒害法篩選市售蜂蜜與嬰兒食品中肉毒桿菌孢子

Combined Methods of Dialysis, Cooked Meat Medium Enrichment and Laboratory Animal Toxicity for Screening Clostridium botulinum Spores in Honey and Infant Food

從1988年7月至1989年4月間，採購全省養蜂場及市販蜂蜜152件，及臺北市超級市場嬰兒輔助食品（包指嬰兒奶粉、麥粉、米粉、混合穀粉及果汁）50件，進行肉毒桿菌孢子之檢測。蜂蜜先行以半透膜法透析，繼之接種於肉羹培養基增菌，然而嬰兒食品則以直接接種於肉羹培養基增菌，其後所有可疑菌落再經Vitek細菌自動鑑別系統，實驗動物毒害試驗法及活體外毒素中和試驗，以進行肉毒桿菌孢子及其毒素之分離與鑑別。試驗結果顯示僅兩件蜂蜜檢出肉毒桿菌孢子，其毒素均為B型；然而所有嬰兒食品均未檢出肉毒桿菌孢子。文中亦探討嫌疑檢體培養液經離心處理後，投予試驗小鼠所呈現之特異性臨床症狀。

One hundred and fifty two samples of honey were purchased from apiaries and markets in Taiwan, meanwhile fifty samples of infant food including powders of infant milk, wheat, rice and commercial mixed cereals, as well as juice had been bought from supermarkets in Taipei city from July 1988 through April 1989. The samples were used for detecting the presence of Clostridium botulinum spores. Honey samples were prepared by dialysis to obtain the bacterial spores; however, infant food had been inoculated directly into the cooked meat medium. The suspicious isolated colonies were identified by Vitek automatic microbial identification system. The supernatants of cooked meat medium enrichment were performed for typing botulinal toxins via the laboratory animal toxicity test. The results showed that two samples of honey contained the spores of C. botulinum type B, but none in samples of infant food. The specific response of clinical signs in mice, after being administered the supernatant of suspected cultural broth, were also observed and described in this article.