| 中文摘要 |
本定量法條採用二甲酚法，將硝酸塩、亞硝酸塩於酸性下分別與，2,6-二甲酚反應，就所得生成物4-硝基-2,6-二甲酚，4-亞硝基-2,6-二甲酚以氯仿抽出後，再利用高效液相層析法分離定量。其高效液相層析條件為：以μ-Porasil （10μm，4mm I.D. x 250mm） 層析柱為固定相，以氯仿─醋酸（99.5:0.5）為移動相，檢出器之紫外光波長為310nm。就市售保色劑及市售肉製品利用本定量法進行檢測，結果以市售保色劑普德粉之配方製成之檢體作回收實驗時，所得平均回收率為硝酸鉀99.1%，亞硝酸納99.54%，至於食品部份，由香腸檢體作回收實驗，所得平均回收率為硝酸塩95.0-101.0%，亞硝酸塩99.0-100.0%，本方法之檢出限量為NO3-N 0.2μg/ml，NO2-N 0.02μg/ml，為一精確迅速、簡便可行之分析方法。 |
| 英文摘要 |
Determination of nitrate and nitrite using xylenol method was studied. It is based on the formation of 4-nitro-2,6-xylenol by the reaction of nitrate with 2,6-xylehol, and the formation of 4-nitroso:2,6-xylenol by the reaction of nitrite with 2,6-xylenol. These two products were extracted with chloroform and simultaneously analyzed by mesans of high performance liquid chromatography with a UV detector (310nm) and a , µ-porasil column with chloroform: acetic acid (99.5: 0.5) as the mobile phase. The recoveries from sausage were 95.0～101.0% for nitrate and 99.0～100.07 for nitrite. The detection limits for nitrate nitrogen and for nitrite nitrogen were 0.2 and 0.02µg/ml, respectively. The methed is rapid and the results were reproducible. |
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