利用氣、液介面細胞株直接暴露系統探討臭氧及β-蒎烯氧化產物對細胞發炎反應之影響

Hazard Exposure of Organic Solvent for Workers in Chloroprene Rubber Adhesives Manufacturing Plants

臭氧是一種擁有強氧化力的氣體，許多研究證實吸入臭氧會引發各種健康問題，包括胸 痛、咳嗽、喉嚨發炎，引發支氣管炎、肺氣腫、哮喘惡化等，臭氧的高反應性也會造成氧化壓 力、增加細胞膜的通透性、造成細胞間反應性氧化物種（Reactive oxygen species, ROS）的增加 甚至會造成細胞的損傷，在許多研究中也已經證實，臭氧與β-蒎烯混合後，會產生甲醛及二 次有機氣膠。

Ozone is a strong oxidant gas. Many studies have showned that ozone can cause a variety of health problems including chest pain, coughing, throat irritation, bronchitis, emphysema, and worsen asthma. The high reactivity of ozone also cause oxidative stress to increase the permeability of the cell membrane, and reactive oxygen species (ROS) that cause cell damage, some studies have also confirmed that the ozone mixed with β-pinene, will produce secondary aerosols and formaldehyde. Our study use air-liquid interface with A549 cells to expose to different concentrations (30,60,120 ppb) at different times (2,4, 6 hours) and the mixture with ozone and β-pinene. We use Trypan Blue to analyze cell viability and use 2'7'-Dichlorofluorescein diacetate (DCFH-DA) with fluorescence enzyme immunoassay analyzer to measure the release of ROS from cells. Polymerase chain reaction (PCR) was to measure the production of inflammatory markers (IL-6, IL-8) after exposure scenario different. The cell viability determined by trypan blue, in the control group were 72% -85%, with the increase of exposure time and ozone concentration, the viability reduced to 52%. From the experimental results of MTT cell activity can also be seen with increasing exposure time and the exposure dose, the absorbance values are significantly lower, the results of statistical also showed cell activity are significant differences in the dose with different exposure concentrations. ROS production in different ozone exposures has shown a dose dependent effects. The production of ROS were significantly increase when expose to the mixtures of ozone and β-pinene at 2 hours. IL-6 and IL-8 also show a dose-dependent effect with increasing ozone and ozone-β-pinene mixures. Our study demonstrate exposure times , levels of ozone and ozone-β-pinene mixture , would trigger a dose- dependant relationships in viability, ROS, IL-6, IL-8. The results of this study also highlight the importance of secondary aerosol and formaldehyde and ofer derivatives from the mixtures of ozone and β-pinene which is one of the common sources of nature fragrance in indoor enviroment.