利用HPLC和Jaffe's測定尿中Creatinine之研究

本研究採用Jaffe’s及HPLC兩種方法，進行尿中肌酸酐的測定。結果顯示，學生之樣本中兩樣分析方法有良好之線性關係，迴歸式：y=10015x + 0.427 （y=HPLC, x=Jaffe’s），r= 0.817，而由醫院所取得之樣本，則兩種分析方法結果則有較大差異。其回歸式：y=0.449x + 6.661，r=0.521，由此可見醫院病人尿液影響因素較多，易對Jaffe’s method產生干擾，進而選擇極端樣品添加回收試驗，其結果：HPLC添加回收率平均值為96.57%、標準差8.795。Jaffe’s method：添加回收率平均值為100.41%、標準差31.616。

In this study, we adopted two methods ‘Jaffe’s and HPLC’ and then detectored creatinine in urine. The result appeared: in the samples of healthy people aged 20-23, both of the methods had excellent line correlation and the regression was y=1.015x+0.427 (y=HPLC, x=Jaffe’s), r=0.817; in the samples from the hospital, there were quite differences between the results of the two methods, and the regression was y=0.449x +6.661, r=0.521. According to this, we can see that there are more interfering factors to the urine of the hospital patients, and the factors are apt to interfere the Jaffe’s method. So, we chose extremely different samples to make a additional recovery test, and we got the result: the average of HPLC additional recovery rate is 96.57%, standard derivation 8.795; the average of Jaffe’s method is 100.41%, standard derivation 31.616.