三甲胺採樣分析方法研發

Development of Sampling and Analytical Method for Trimethylamine

三甲胺為工業上使用甚廣之原物料，且為法規所管制者，但國內外目前尚無參考分析方法可用於監測作業環境中勞工之暴露程度。本研究建立之三甲胺採樣分析方法係利用酸化濾紙來捕集，並利用固相微萃取及GC/FID 進行分析。測試結果顯示，三甲胺的檢量線範圍在0.04 至0.8 mg 間；方法偵測極限為2.98 μg；平均脫附效率為100.4%；以100 ml/min 採樣時，破出時間大於4 小時，推求出本方法之建議最大採樣體積為16 升。在精密度上求出之分析變異係數為2.11%，而總變異係數為3.4%。且求得之平均採集效率為105.1%。在28 天之測試期間內，室溫及冷藏兩種儲存條件的回收率均可達到100±10%之要求。總合而言，本研究測試的三甲胺檢測方法，除使用之材料與儀器簡便可行外，亦能符合驗證準則中之測試規範，達到一定之準確與精密度，因此，可作為國內制定三甲胺參考分析方法之依據。

Trimethylamine is commonly used in various industries, therefore the potential for occupational exposure exists. Trimethylamine is currently regulated with permissible exposure level but there is no reference method to monitor this compound in the workplace. In current study a new approach, which utilizes acidified filter in sampling, solid-phase microextraction (SPME) in extracting and GC/FID in analysis, was developed and evaluated for monitoring airborne trimethylamine. The results indicate that the mean collection efficiency of the acidified filter is 105.1% and the breakthrough volume is 24 liters, based on a sampling rate of 100cc/min. and concentration at 2 times the recommended exposure level. The extraction is completed within 5 minutes under a non-equilibrium condition, using PDMS fiber as extracting media. The calibration range is 0.04-0.8mg and the mean desorption efficiency is 100.4%. The analysis coefficient of variation and the total coefficient of variation is 2.11% and 3.4%, respectively. Samples are stable for at least 28 days under both ambient and freeze conditions. In short, this method is simple, feasible and with satisfactory accuracy and precision, and thus can be used as a reference method to monitor occupational exposure to trimethylamine.