鈷、鉻金屬與人體血清白蛋白體外反應結合性質之探討

Investigation of In Vitro Binding Reaction between Cobalt/Chromium Particles and Human Serum Albumin

本研究探討鈷、鉻等二類金屬與人體血清白蛋白之吸附容量及結合性質，並探討金屬結合對人體血清白蛋白分子構形之影響。其中吸附容量及結合性質之判定，以吸附等溫線測定來進行；而蛋白質構形變化則以圓二色旋光度（CD, Circular Dichroism）之測定進行評估。結果顯示血清白蛋白與鈷、鉻之結合可能屬多層吸附，其對鈷之結合乃一放熱反應，而其與鉻之結合則為一吸熱反應》另外，此二金屬與人體血清白蛋白之結合造成血清白蛋白分子二級結構之變化。

The binding reaction of human serum albumin onto cobalt or chromium particles is investigated in this study. The binding capacity and other binding properties were analyzed by adsorption isotherms. The conformational change of the protein was assessed by circular dichroism spectroscopy. Results showed that the binding of human serum albumin onto the cobalt particle was an exothermic reaction, while its binding onto the chromium particle was an endothermic one. The maximum adsorption of serum albumin onto cobalt or chromium particles seemed to form multilayers. And the adsorption led to the change in the secondary structure of the serum albumin.