Background: Increasing use of complementary and alternative medicine (CAM) by the public has drawn major concern from healthcare professionals. To provide better care, it is vital that nursing students are prepared to face these new trends. Prior to further planning, it is necessary to assess students' knowledge and beliefs toward complementary and alternative medicine. Objectives: The study aimed to explore the nursing students' knowledge, belief, and use of complementary and alternative medicine. Design: This is a cross-sectional descriptive study. Setting: The study was performed in one university of north Taiwan. Participants: The participants of the study are nursing students in their last semester prior to graduation. Methods: A purposive sampling was used and utilized self-reported questionnaires to collect the data. Tools included knowledge of CAM, the Complementary and Alternative Medicine Health Belief Questionnaire-Chinese Version, and a checklist of CAM used in Taiwan. SPSS 17.0 was used for the statistics. Results: A total of 300 female and single nursing students enrolled in this study. Participants mean age were 21.42, about half of those students (49.7%) held some religion. Nearly three quarters (75.7%, n = 227) come from nuclear family. Participants rated their knowledge about CAM relatively low (in a 1-5 range, mean scores were 1.36-2.39). Scoring lowest was knowledge in alternative medicine (Mean = 1.36; SD = 0.66). Knowledge in mind-body interventions scored highest (Mean = 2.39; SD = 0.68). The students' belief toward complementary and alternative medicine achieved a mean score of 45 (SD = 5.51), which indicated that they held positive belief toward it. A total of 147 nursing students (49%) reported past or present use of at least one complementary and alternative therapy per month. The results showed that belief had a significant relationship with knowledge in the five category modalities and with personal use (p ＜. 05). The knowledge of CAM also had positive correlation with personal use. (except the alternative medicine system). Conclusions: The findings highlight the strong relationship between knowledge, belief, and personal use of CAM. The findings imply the need for and the importance of integrating CAM into nursing education curriculums. Nursing education programs should focus on these important elements of the future.