This study investigated the relationship between practicum-related stress and sleep quality in healthcare administration students. Researcher recruited a total of 113 healthcare administration students currently taking a practicum course as study participants. The questionnaire was composed of three parts: the basic information and a Stress Scale and Pittsburgh Sleep Quality Index (PSQI). The results showed that students perceived a mild level of stress (1.87). The major stressor was practice report and homework, followed by worrying about insufficient professional knowledge and skills, and practice workload. Participants slept an average 6.42 hours per day (SD = 0.84). The average participant PSQI score was 6.70 (SD = 3.52). A large majority (52.2%) of participants reported poor sleep quality. Researcher was found practicum-related stress significantly correlated with hours of sleep and sleep quality of the daytime dysfunction (p ＜ .01). Results of multiple regression analysis showed that health condition during the internship period, the interest in healthcare administration were significant predictors of sleep quality, and accounted for 32.5% of total variance. Study findings may help healthcare administration teachers understand practicum-related stress and the impact of such on sleep among healthcare administration students currently taking their practicum, and to provide assistance and counseling to students of healthcare administration.