| 中文摘要 |
惡性腫瘤是國人十大死亡原因的首位，大腸癌躍居癌症死亡的第三位。有文獻報導大腸癌的復發或轉移和基質金屬蛋白{^FAF0^} MMP-2 、MMP-7 和MMP-9 的過度表達有正相關。黃耆皂{^FAF5^}Ⅳ （astragaloside Ⅳ ）是黃耆之主要成分。本研究結果發現黃耆皂{^FAF5^}Ⅳ 在濃度10~100 ng/mL 作用12~48 小時皆可促進人類大腸癌HT-29 細胞株的生長，尤其在50 ng/mL 時更明顯。研究結果亦發現MMP-2 mRNA 在黃耆皂{^FAF5^}Ⅳ 濃度10 及20 ng/mL 時有明顯表達，但在濃度50~100ng/mL 時則有抑制的作用。黃耆皂{^FAF5^}Ⅳ 在濃度20~100 ng/mL 時TIMP-1 mRNA 有明顯表達，而MMP-7 、MMP-9 及TIMP-2 皆沒有表達。Malignant tumor (cancer) is the most important disease of the top ten causes of death in Taiwan. Among cancer types, colon cancer is the third most common cause of cancer-related mortality. The metastases and recurrent rate of colon cancer was correlated with the mRNA level of Matrix Metalloproteinase (MMP). AstragalosideⅣ is one of the important constituents of Huang-qi. In the present study, our results showed that the cell proliferation rate of HT-29 was stimulated by treatment with Astragaloside Ⅳ at 10-100 ng/mL for 12-48 hrs, especially high at 50 ng/mL. The mRNA level of MMP-2 was elevated by Astragaloside Ⅳ at 10 and 20 ng/mL. However, this level was decreased by Astragaloside Ⅳ at 50 and 100 ng/mL. The mRNA level of TIMP-1 was increased by Astragaloside Ⅳ at 20-100 ng/mL. In contrast, the mRNA levels of MMP-7, MMP-9, and TIMP-2 were not detectable in HT-29. |
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