電針對食源性肥胖大鼠血中瘦體素與胰島素的影響

EFFECT OF ELECTROACUPUNCTURE ON PLASMA OF LEPTIN AND INSULIN IN DIET-INDUCED OBESE RATS

過去文獻指出，針刺對減肥有一定的療效，但其詳細的作用機轉則不明確。本研究的目的 是以100 Hz 電針來刺激大鼠兩側足三里穴及三陰交穴，觀察其對食源性肥胖大鼠的體重、攝 食量、血脂、瘦體素（Leptin ）與胰島素（Insulin ）的影響。使用方法是將餵食高脂飼料造模成 功的DIO （diet-induced obesity ）雄性Wistar 大白鼠，任意分為實驗組及對照組，實驗組是先予 以固定於自製圓形塑膠瓶內，然後再加以100 Hz 電針，每天刺激雙側足三里穴及三陰交穴三 十分鐘，連續治療14 天，對照組則如同實驗組予以固定於自製圓形塑膠瓶內但不電針。結果 顯示：在持續餵食高脂飼料下，連續治療14 天後，對照組體重及Lee’s index 均有明顯的增加， 但是實驗組則沒有明顯的增加。進一步分析體內血脂變化則發現實驗組的血中三酸甘油脂（TG ） 會明顯的下降、血中高密度膽固醇（HDL ）也明顯的上升，而對照組則否。此外，對照組血中 瘦體素與胰島素的濃度會明顯的上升，但實驗組則沒有。因此，100 Hz 電針刺激在食源性肥胖 大鼠的足三里穴及三陰交穴，對持續進食高脂飲食的食源性肥胖大鼠具有預防體重上升的效 果，並且，能降低其血中的三酸甘油脂，可提供臨床上治療食源性肥胖症的參考。It has been reported that electroacupuncture (EA) is effective in reducing body weight. However, its mechanism was unclear. The purpose of this study is to illustrate the effect of electroacupuncture, which stimulates bilateral Zusanli and Sanyinjiao acupoints, on body weight, food intake, plasma lipid, plasma leptin, and plasma insulin in diet-induced obese (DIO) rats. The DIO male wistar rats were randomly divided into two groups, one is electroacupuncture (EA) group and the other is control group. EA group was treated with 100 Hz EA on bilateral Zusanli and Sanyinjiao acupoints for 30 minutes once a day for 14 days. Control group is restrained as the EA group, without being treated with EA. After 14 days, our results showed that body weight and Lee’s index were increased significantly in control group, but not in EA group. In addition, the plasma content of TG is decreased and HDL is increased significantly in the EA group, however, the control group shows no change. The levels of plasma leptin and insulin were increased significantly in control group, but not in EA group. In summary, 100 Hz EA on bilateral Zusanli and Sanyinjiao acupoints can inhibit the weight gain of DIO rats with high fat diet and can decrease the plasma triglyceride.