當歸補血湯抽出物促進人類周邊血單核細胞之細胞增殖與細胞激素產生

Enhancement of Cell Proliferation and Cytokines Production in Human Peripheral Blood Mononuclear Cells by Extracts from Blood-Enriching Dang-Gui Decoction

當歸補血湯（Blood-enriching Dang-Gui Decoction ）是由當歸（Angelica sinensis ）與黃耆（Astragalus membranaccus ）所組成，在傳統中醫上用於促進人體健康，在此研究中我們將之用於免疫藥理活性分析。我們由當歸補血湯、當歸與黃耆中各別取得水抽出物，將之分別加入人類周邊血單核細胞（Human peripheral blood mononuclear cells ）中，以放射線氚- 胸嘧啶吸收法（3H-Thymidine Uptake ）分析細胞增殖的情形，結果顯示當歸補血湯、當歸與黃耆各別水抽出物皆能促進植物凝集素（Phytohemagglutinin ）所引起之細胞增殖，它們促進細胞百分之五十增殖之濃度（EC50 ）分別為106.3 ± 5.8 μg/ml, 91.3 ± 2.5 μg/ml 及23.8 ± 4.0 μg/ml 。當歸補血湯、當歸與黃耆其促進人類單核細胞增殖之作用機轉可能與調控第二介白質（Interleukin-2; IL-2 ）、第三介白質（Interleukin-3; IL-3 ）及丙種干擾素（Interferon-γ; IFN-γ ）產生有關，因為當歸補血湯、當歸與黃耆各別水抽出物皆能促進植物凝集素（Phytohemagglutinin; PHA ）所引起之第二介白質、第三介白質與丙種干擾素在人類周邊血單核細胞中之產生，並呈一劑量性反應。因此我們認為，在當歸補血湯中含有免疫調控因子（Immunomodulatory agents ）。“Blood-enriching Dang-Gui Decoction” (BDGD), comprised of Angelica sinensis (Oliv.) Diels and Astragalus membranaccus (Fisch) Bge and used for improvement health in traditional Chinese medicine, was selected for immunopharmacologica1 activity test. Effects of aqueous extracts of BDGD, A. sinensis , and A. membranaccus on human peripheral blood mononuclear cells (PBMC) proliferation were determined by tritiated thymidine uptake, respectively. Aqueous extracts from all three preparations significantly enhanced the proliferation of PBMC induced by phytohemagglutinin (PHA) with an EC50 of 106.3 ± 5.8, 91.3 ± 2.5, and 23.8 ± 4.0 μg/ml, resepctively. Furthermore, all extracts dose-dependently enhanced interleukin-2 (IL-2), interleukin-3 (IL-3), and interferon-γ (IFN-γ ) production in PHA-stimulated PBMC, suggesting that pharmacological activities of these drugs may be mediated by regulating the production of cytokines in PBMC. These results indicate that agents with immunomodulatory effects are contained in BDGD.