風藤抗B 型肝炎病毒活性成分之研究

Anti-Hepatitis B Virus of Seven Compounds Isolated from Piper kadsura (Choisy) Ohwi

以具有 B 型肝炎病毒基因體嵌入其內之人類肝癌細胞株MS-G2 ，進行中草藥抗病毒之篩檢。Futoquinol 、Futoenone 、（+）-Crotepoxide 、（-）-Galbelgin 、meso-Galgravin 、Piperlactam S 及Piperenone 為七個分離自胡椒科（Piperaceae ）植物風藤Piper kadsura （Choisy ）之純化合物，實驗結果顯示：Futoquinol, （-）- Galbelgin 及meso-Galgravin 三個成分，均於25 μM 濃度具有顯著抑制B 型肝炎病毒表面抗原及e 抗原之活性，Piperenone 對B 型肝炎病毒表面抗原無抑制作用，須要到高劑量50μM 才對e 抗原具輕度抑制作用，至於Piperlactam S 其對B 型肝炎病毒的有效作用濃度（EC50 ）為20.6 ± 0.7 μM ，對MS-G2 細胞生長抑制濃度（IC50 ）為23.9 ± 3.4 μM ，顯示Piperlactam S 無抑制病毒的作用，而具細胞毒性作用，而其他二個成分Futoenone 及（+）-Crotepoxide 則無抑制B 型肝炎病毒表面抗原及e 抗原之活性。In Vitro anti-human hepatitis B virus effects of Chinese and folk herbal drugs on the HBV producing cell line (MS-G2) were studied. Among seven compounds isolated from Piper kadsura (Choisy) Ohwi, we found that futoquinol, (-)-galbelgin, and meso-galgravin at concentration of 25 μM can effectively suppress both HBV surface and e antigen productions, and high concentration (50 μM) of piperenone can only suppress HBV e antigen productions, while piperlactam S even depicts significant cytotoxic effects. In contrast, futoenone and (+)-crotepoxide do not show any noticeable anti-viral effects.