醫林改錯活血化瘀方劑對活動力正常精子之體外強精效應

The Essence-Strengthening Effect of Huo Xue Hua Yu Formulas on Normal Sperm Motility in Vitro

根據「精血同源，血能化精」的中醫理論，本實驗以清朝王清任醫林改錯書中之著名活血化瘀三種方劑，觀察其對人類正常活動力精子的體外強精效應。實驗以電腦輔助精液分析法，分析十六個精液標本之精子活動力與前進活動力；實驗分組三種方劑溶液為實驗組，包括血腑逐瘀湯、膈下逐瘀湯和少腹逐瘀湯，每個方劑溶液分1.0，0.1和0.01mg/ml 等三種濃度，對照組分未加任何藥之精液組與加 F-10 溶液之精液組。實驗結果，三種方劑溶液對正常精子活動力的體外強精效應，在實驗 120 分鐘時出現非常顯著的強精作用，且在精子前進活動力方面，包括精子游動線性指標比值和精子游動路徑常態速率均有促進作用，因此這些方劑在生殖科技的體外受精應用上，將可提供開發的參考。

On the basis of the Chinese medical theory of that 'blood and essence are of the same source and blood can transform into essence', we selected three well-known Huo Xue Yu (blood-quickening stasis transforming) formulas originating from the Yi Lin Gai Guo ('Correction of Medical Errors') by Wang Qing Ren in the Qing Dynasty, in order to observe their effect on normal sperm motility in vitro. We analyzed 16 semen samles using the computer-assisted semen analyzer. We divided samples into an experimental group and a control group. In the experimental group, three solutions of XueFuZhu Yu Tang, GeXiaZhuYu Tang, and Shao Fu Zhu Yu Tang formulas, at three different concentrations of 1.0, 0.1, and 0.01 mg/mL. The control group contained semen samples without the addition of any herbs, and semen samples with addition of F-10 solution. The results show that the essence-strengthening effect of three formulas on low sperm motility in vitro is markedly significant for a duration of 120 minutes. These formula solutions promoted normal progressive sperm motility including straightness and path velocity (VAP). In conclusion the action of these formulas in increasing sperm motility and forward progression may be further study on reproductive technology.