欖仁樹鮮葉、陰乾鮮葉及乾燥落葉所含Punicalin及Punicalagin之高效液相層析法定量分析

Determination of the Contents of Punicalin and Punicalagin from Fresh Leaf, Dried Leaf and Dried Fallen Leaf of Terminalia Catappa L. by HPLC

欖仁樹為民間藥，含有許多加水分解型鞣質。依其使用部位的不同，有不同的治療用途，如：葉、果實、種子、樹皮等，分別作為止瀉、解熱、止血、收斂等藥用。台灣民間使用其落葉經乾燥後，用於肝病的治療。本文利用高效液相層析法定量其陰乾鮮葉，鮮葉及乾燥落葉之水抽出物中所含punicalin及punicalagin的含量。結果發現陰乾鮮葉含量最高：punicalin (0.15%)，punicalagin(0.027%)，鮮葉次之：punicalin (0.046%)，punicalagin (0.0044%)，乾燥落葉最低：punicalin (0.029%)，punicalagin (0.0013%)。另鮮葉，陰乾鮮葉及乾燥落葉之水抽出物產率分別為5.9%，9.6%及9.8%。

Tea brewed from the fallen leaves of T. catappa L. which has many hydrolyzable tannins is used as folk medicine to treat liver disorder in Taiwan. An HPLC method was used to examine the contents of punicalin and punicalagin from samples of fresh leaf, dried leaf, and dried fallen leaf of T. catappa L. Leaves were twice boiled in water over a period of 10 min. prior to HPLC analysis. The extraction rate was 5.9%, 9.6% and 9.8% for fresh leaf, dried leaf and dried fallen leaf, respectively. The results showed that the amount of punicalin and punicalagin in the samples assessed were: 0.046%; 0.0044% (fresh leaf), 0.15%; 0.027% (dried leaf), and 0.029%; 0.0013% (dried fallen leaf).