玉山龍膽之成分及其降血壓效果

Characterization of Constituents in Gentiana Scabrida and Their Hypotensive Effect

為繼續尋求臺產龍膽科活性物質，因此又從玉山龍膽(Gentiana scabrida)的甲醇抽出物經矽膠柱層層析法分離得一種屬triterpenoid ester之共存同分異構混合新物質，28-acetyl urs-12-en-3β，28-diol 3-palmitate與28-acetyl olean-12-en-3β，28-diol 3-palmitate，其他還分離證明得三種共存問分異構混合物，α-amyrin palmitate與ß-amyrin palmitate，urs-12-en-3β，28-diol 3-palmitate與olean-12-en-3β，28-diol 3-palmitate 及urs-12-en-3β，28-diol與olean-12-en-3β，28-diol；lupeol 3-palmitate；uvaol；β-sitosterol；uroslic acid；2α-hydroxyursolic acid；β-sitosterol-β-D-glucoside；quercetin；quercetin-3-O-galactoside；quercetin-3-O-(6-O-α-L-arabinopyranosyl)-ß-D-galacto-pyranoside；及mangiferin等十三種已知物質。因文獻上報告flavonols具有降血壓作用，因此由玉山龍膽分離得之flavonols，進行降血壓活性試驗發現quercetin-3-O-(6-O-α-L-arabinopyranosyl)-β-D-galactopyranoside對麻醉之大白鼠具有顯著地降血壓活性，而quercetin及querctin-3-O-galactoside對麻醉之大白鼠並無顯地降血壓活性。

A new, mutually isomeric mixture of triterpenoid esters, 28-acetyl urs-12-en-313, 28-diol 3-palmitate and 28-acetyl olean-12-en-3B, 28-diol 3-palmitate, and thirteen known compounds have been isolated from the fresh whole plant of Gentiana scabrida Hayata (Gentianaceae) and characterized by spectroscopic methods and chemical reactions. Pharmacological studies on the flavonols isolated from this plant, quercetin-3-O-(6-O-α-L-arabinopyranosyl)-β-D-galactopyranoside showed significant hypotensive activity.