中藥純化物對陰道滴蟲作用的研究

Stimulatory Effect of Furoquinoline Alkaloids on the Growth of Trichomonas Vaginalis

為了想要瞭解天然物純化成分對陰道滴蟲可能產生的影響以利開發新的治療藥，本項研究使用由陳益昇教授（高雄醫學院）抽自山刈葉(Evodia merrilli)的三種furoquinoline生物鹼：茵芋素(skimmianine)、康服素(confusameline)和庫沙素(kokusaginine)來探討它們對培養的陰道滴蟲可能產生的影響。經過30個小時在37ºC恒溫的培養，三種純化成分皆有刺激陰道滴蟲成長的作用；以茵芋索和庫沙素的刺激作用讓蟲體成長較早達到最大的效果。由作用的濃度來比較的話，茵芋素的作用最強，康服素次之，庫沙素則最弱。由於蟲體的蛋白質含量也同時有增多的現象，刺激蛋白質的生合成似乎與這項效果有關。因此，本次所篩選的三個純化物對陰道滴蟲並無直接抑制作用。瞭解本次刺激現象的產生原因，一則可深入解明陰道滴蟲的成長過程來提供尋找阻斷的方法，更可幫助我們探尋產生刺激效果的官能基來加予化學修飾，或許可將它們由刺激作用轉變為抑制功能，協助臨床開發新的陰道滴蟲感染治療藥。

Effects, of three furoquinoline alkaloids, extracted from the leaves of Evodia merrilli (Rutaceae), on the growth of Trichomonas vaginalis were investigated. After 60 hours incubation with skimmianine, growth of Trichomonas vaginalis became more active markedly. Also, Kokusaginine and confusameline stimulated the growth of this parasite in a dose-dependent manner. Protein contents of this parasite were raised by the furoquinolines indicating the participation of protein synthesis. Comparison of the stimulatory activities, they act in the order of skimmianine, Confusameline and kokusaginine. The obtained results suggest that furoquinoline alkaloids possess the ability to stimulate the growth of cultured Trichomonas vaginalis.