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Objective
High purine diet was a main reason for hyperuricemia and the patients might have renal impairment. This study aimed to study the effect of a high-purine diet on hyperuricemic nephropathy and investigate the molecules involved in hyperuricemic nephropathy in rats.
Methods
38 male rats were randomly divided into control group and hyperuricemia group. Renal function of the rats was measured by levels of serum uric acid (UA), urea nitrogen (BUN), creatinine (CRE), creatinine clearance rate (Ccr), 24 hour microalbuminuria (MAU) and LKW/BW (left kidney weight / body weight). Histological changes in the kidney were detected by Hematoxylin-eosin (HE) and MASSON staining. The levels of IL-1β, interleukin-1 receptor-associated kinase4 (IRAK-4) and Matrix Metallopeptidase 9 (MMP-9) were measured by immunohistochemistry or real-time PCR.
Results: The level of UA (P=0.003), BUN (P=0.008), CRE (P=0.002), MAU (P=0.002) and LKW/BW (P=0.039) were higher in hyperuricemia group than control group, while the level of Ccr (P=0.001) was lower. Compared with control group, hyperuricemia rats showed impaired renal function and interstitial fibrosis. The expression of IL-1β, IRAK-4 and MMP-9 was significantly increased (P=0.002; P=0.001).
Conclusion
A high-purine diet was the main reason for hyperuricemia and damage to renal function. The expression of IL-1β was higher in hyperuricemic nephropathy, which was closely related to the expression of IRAK-4 and MMP-9.