| 英文摘要 |
Antibiotic-resistant Acinetobacter baumannii is a very important cause of healthcare-associated infections. Being highly pathogenic and having high mortality, it is one of the major healthcare-associated infecting pathogens. In addition to plasmid and transposon, integron is the genetic unit that confers antibiotic resistance to bacteria. In this study, 100 A. baumannii clinical blood culture isolates were collected from Show Chwan Memorial Hospital, Changhua, Taiwan from 2008 to 2010 and their antimicrobial susceptibility against 14 kinds of antibiotics were examined. Results showed a significant increase (from 54% to 66%) in drug resistance of A. baumannii to cephalosporins. An upward trend in resistance to PIP, TZP, SXT and SAM was also observed. In contrast, there was a marked decrease (from 60% to 48%) in drug resistance to aminoglycosides.
Moreover, the CS-PCR method was employed to detect the existence of class 1 integron in these isolates. It was found that 61.8% of these isolates carried the class 1 integron, and the cassette arrays they carried were of four different sizes: 3.0 kb, 2.5 kb, 1.5 kb and 1.0 kb, with the majority of isolates (87.3%) carrying the 2.5 kb cassette array. Results also showed that integron-carrying A. baumannii isolates were more resistant to various antibiotics tested than non-integron-carrying isolates. The widespread occurrence of 2.5 kb cassette array in A. baumannii isolates from the case hospital was rarely reported in other countries.This phenomenon may be linked to the hospital’s preference of certain antibiotics, causing the A. baumannii strain carrying the 2.5-kb cassette array to become the dominant antibiotic being administered. |
