Regular treatment sessions at hospitals are required for patients on hemodialysis. As a result of the ongoing COVID-19 pandemic, inadequate protective measures can easily lead to a cluster infection. This project was conducted from February 23rd through September 30, 2021, during which several issues were identified regarding the COVID-19 safety measures implemented by nurses in hemodialysis units, including: the absence of an audit framework and in-service training programs at the unit; the nurses’ poor understanding of the procedures for putting on personal protective equipment, hemodialysis precautions, and patient isolation flow; and the lack of terminal disinfection in the hospital environment and equipment. To address these issues, an integrated flipped learning strategy was applied to develop audit frameworks, train seed teachers, organize in-service training programs and drills, create occupant flow diagrams, and devise terminal disinfection protocols. Following the implementation of this project, the hemodialysis nurses’ knowledge of COVID-19 safety measures increased from 57.3% to 96.8%, and their accuracy in implementing safety measures increased from 58.1% to 92.5%.The results of this study are serve as a reference for hemodialysis nurses to provide care to patients with novel infectious diseases, and facilitate the development and implementation of epidemic prevention policies that ensure safety in the healthcare system.