擴增台灣本土幽門螺旋桿菌質譜儀系統資料庫

Optimizing of MALDI-TOF Dat abase for Helicobacter pylor

以基質輔助激光解吸電離-飛行時間質譜(matrix-assist ed laser desorption ionization-time of flight mass spectrometry, MALDI-TOF MS)是一種方法簡單、快速、成本低且能準確鑑定細菌與真菌菌名的儀器，近年來廣泛使用在臨床微生物實驗室鑑定。但對於幽門螺旋桿菌(Helicobacter pylori)鑑定，受限於資料庫不足，導致臨床實驗室無法運用此種方法。本研究使用經定序確認的100株臨床幽門螺旋桿菌菌株，建構質譜圖與親緣性樹狀分析圖，發現27型不同於Bruk er資料庫既有的7個型別，我們將此27型建入既有資料庫，使用另外20株臨床菌株，進行4重複鑑定進行資料庫驗證。結果顯示19株鑑定值皆可>2.0，唯有一株其結果為1.7分。本實驗所擴增的資料庫，可大幅提高鑑定分數，有助於未來運用於臨床幽門螺旋桿菌鑑定。

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an accurate, and cost-effective method used for identifying microorga nisms but its application was limited for identifying Helicobacter pylori (HP) for insufficient databases. In this study, we used previously sequenced 100 clinical HP isolates to generate mass spectra using MALDI-TOF M S. We used Flex Analysis and MALDI Biotyper 3 to plot phylogenetic trees and found 27 different types in ad dition to the 7 types in the Bruker database. These 27 types were added to the existing database, which resulte d in a significant increase in identification scores. We further tested the updated database using 20 other clini cal HP isolates, and the identification results were highly accurate (95.0%). Our establishment of the c omprehensive database significantly improved the accuracy of identifying HP using MALDI-TOF MS in the future.