Environmental cleaning is key in preventing and controlling health care–associated infections. First, environmental cleaning can reduce the survival and reproduction of pathogens, especially those of common drug-resistant bacteria. The regular cleaning and disinfection of high-risk areas, such as wards, operating rooms, and nursing stations, can effectively reduce bioburden levels. An excellent cleaning environment reduces the risk of medical staff and patients being exposed to pathogens, thereby reducing the incidence of medical care–associated infections. Cleanliness is a goal of disease safety and an strategy for implementing infection control. Since 2017, various stipulations pertaining to the monitoring of environmental cleanliness quality have been implemented. The implementation of environmental cleaning and effective monitoring are crucial aspects of infection control and prevention. At present, the methods commonly used to monitor the quality of hospital environmental cleanliness can mainly be divided into three categories, namely visual observation, fluorescent labeling and detection, and microbial load residue detection. Numerous studies have explored the aforementioned environmental cleaning and monitoring methods and reported that they improved the quality of environmental cleaning by cleaning personnel and reduced bacterial colonization and the spread of bacterial infections or contamination. Accordingly, health care facilities must strengthen their environmental cleaning processes and conduct regular cleaning and disinfection to reduce the survival and reproduction of pathogens. These measures reduce the risk of patients contracting pathogens and the risk of resistant strains spreading.