應用24個STR基因座──Globalfiler Kit於親子鑑定之成本效益與臨床應用評估

Assessment of 24 Short Tandem Repeat Loci of Globalfiler Kit in Paternity Testing

本研究評估24個STR基因座試劑Globalfiler Express PCR Amplification Kit於親子鑑定之成本效益、臨床應用，以符合Taiwan Accreditation Foundation (TAF)之親緣DNA鑑定技術規範，將累積親子關係似然率提高到10000。分析48對原使用Identifiler Kit之親子鑑定檢體：32對似然率小於4000、8對排除數小於5、8對點突變。使用Globalfiler Kit後，29對(90.6%)似然率大於10000，7對(87.5%)排除數大於5，8對點突變似然率增加。研究顯示：此試劑改善親子鑑定之累積親子關係似然率、排除數，36對(75.0%)不需加做，大幅降低試劑、人力成本，改善檢驗時效。

To satisfy the requirement of Taiwan Accreditation Foundation (TAF) with the adaption of combined likelihood ratio (CLR) higher than 10000 in paternity testing, we evaluate the cost- effectiveness and clinical value of 24 short tandem repeat loci of Globalfiler Kit in paternity testing. 48 ambiguous paternity cases assessed by Identifiler Kit in 2013 including 32 duos with CLR＜4000, 8 duos with exclusions ＜5 and 8 duos with point mutation were reanalyzed by Globalfiler Kit. By using the Globalfiler Kit, CLRs of 29 cases were elevated than 10000 (90.6%, 29/32), and 7 cases became excludable. Moreover, the CLRs of 8 duos with point mutation were increased significantly. Most importantly, 75% (36/48) cases meet the criteria by CLR ＞10000 and exclusions ＞5 in paternity testing. In conclusion, the turnaround could be shortened and the cost could be reduced in paternity testing by using the Globalfiler Kit.