Objective: This paper explores the participation of a hospital in cerebrovascular accident disease care certification and its effect on the care of patients with stroke. Throughout the certification process, in which the stroke team strengthened the operation process(standard operating procedure) and engaged in quality improvement activities, acute stroke hospital quality performance measurement index(P4P) were employed to monitor the effectiveness of care for patients with acute stroke. Materials and methods: This study was conducted on patients with acute stroke at a medical center in Taipei, Taiwan. The hospital applied for certification in May 2019. Data collection proceeded from January 2017 to December 2019. The control, intervention, and effect maintenance groups comprised 566, 330, and 216 individuals, respectively. Results: A comparison of the P4P before and after the certification and effect maintenance indicated that the following indicators have been significantly improved: stroke scale assessment completion rate, rate of intravenous tissue plasminogen activator being injected into acute stroke patients in emergency department within 3 hours, related drugs use rate, the rate of patients receiving rehabilitation assessment or treatment. In addition, the number of intra-arterial thrombectomy performed by the team has increased. Conclusion: The participation in the certification process, team resource integration, and process improvements increased the effectiveness of the care of patients with stroke.