This research is a continuation of the previous study done by the researcher that inves-tigated special education pre-service teachers’ learning experiences of sign language. The purpose of this case study was to explore the professional growth of those pre-service teachers (PSTs) who used sign language during their deaf education practicum. In this study, an university advisor, and practicum supervisors from a special school cooperated in arranging a nine-week field practicum for 24 PSTs to teach Chinese in either a junior high or high school division of a special school. The research used the framework of the interactive teaching model and teaching indicators. The PSTs (1) adopted the model of group cooperation in discussing instruction curriculum, teaching strategies, and practicing sign languages; (2) embraced lesson plans, teaching activities, and sign languages practices guided by their practicum supervisors; and (3) engaged in peer observation feedbacks and self-reflection on teaching. The result of this study found: (1) the PSTs sensed tension and felt that it was a challenging task at the very beginning; however, at the end, they believed that they had made improvements after teaching the subject with the sign language; (2) in the aspect of their performance on pedagogical content knowledge, seven dimensions of teaching indicators had shown positive growth; and (3) the cooperation between special education teacher preparation program and special schools strengthened the classroom interactions between PSTs and stu-dents with hearing impairment, which contributed to PSTs’ professional growth of teaching.