運用三磷酸腺苷檢測高壓氧艙體清潔之確效性

Using Adenosine Triphosphate Assay to Detect the Effectiveness of Cleaning of Hyperbaric Oxygen Chambers

近年來全世界受到嚴重特殊傳染性肺炎疫情的影響非常巨大，此病毒可通過多種途徑傳播。環顧國內外針對高壓氧艙體，皆未設定出完整性的清潔規範。本研究透過ATP生物冷光反應法(adenosine triphosphate bioluminescence technique)為環境清潔稽核工具，以清水、低濃度漂白水、Steridal Solution 0.5%(Chlorhexidine)及ATK Antiseptic and Sterilizing Solution，分析四種清潔劑對菌數改變之差異性為何。結果顯示：促使菌數下降最為顯著依序為，Bleach清潔後可立即達到最佳效果(p<0.001)，其次是Chlorhexidine 0.5%第二次清潔後(p<0.01)，而ATK於第二次清潔後，對於菌數的改變並不大。因此如高壓氧艙這密閉式環境清潔，本研究建議可利用Bleach作為首要清潔，可顯著性的降低細菌數，確實提供病人安全的治療環境。

In recent years, the world has been greatly affected by severe special infectious pneumonia epidemics. This virus can be spread through multiple ways. Looking around at home and abroad, no complete cleaning standards have been set for hyperbaric oxygen chambers. Therefore, this study uses ATP bioluminescence technique (adenosine triphosphate bioluminescence technique) as an environmental cleaning audit tool, and uses clean water, low-concentration bleach, Steridal Solution 0.5% (Chlorhexidine) and ATK Antiseptic and Sterilizing Solution to analyze the differences in bacterial counts among four cleaning methods. The results showed that the most significant reduction in bacterial count was achieved immediately after cleaning with Bleach (p<0.001), followed by Chlorhexidine 0.5% after the second cleaning (p<0.01), and ATK after After the second cleaning, the bacterial count did not change much. Therefore, for the cleaning of closed environments, such as hyperbaric oxygen chambers, this study suggests that Bleach can be used as the primary cleaning, which can significantly reduce the bacterial count and truly provide a safe treatment environment for patients.