| 英文摘要 |
Bongkrekic acid (BKA) poisoning is a severe foodborne illness with a high mortality rate. This study aimed to identify BKA in postmortem tissues from poisoning victims in Taiwan and to develop a reliable analytical method for detecting BKA in biofluids to aid clinical diagnosis and treatment. BKA was identified in postmortem samples using high-resolution mass spectrometry (HRMS) and confirmed with an independent ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) method. To meet clinical needs, a UHPLC-MS/MS method was developed and validated for BKA detection in plasma and urine. Method optimization included adjustments to ion source conditions for multiple reaction monitoring (MRM) transitions and avoidance of glass vials due to BKA adsorption onto free silanol groups. Chromatographic separation was achieved using a 50-mm Hypersil Gold C18 column within a 6-min run time. The validated UHPLC-MS/MS method successfully detected BKA in biofluids, enabling its application in identifying victims of foodborne poisoning. The method demonstrated high accuracy and efficiency, facilitating timely diagnosis and aiding in treatment strategies for critically ill patients. The developed UHPLC-MS/MS method provides a reliable approach for detecting BKA in clinical and forensic settings. Its implementation enhances diagnostic capabilities, improves patient outcomes, and supports monitoring of toxin elimination in cases of BKA poisoning. |
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