茶葉中摻加雞母珠小葉或根之鑑別方法   全文下載

Method of Identification for Rosary Pea Leaflets or Root in Tea Leaves

豆科（Fabaceae）植物雞母珠Abrus precatorius L.之乾燥小葉在民間常做為草藥或茶飲使用，惟其使用部位與衛生福利部食品藥物管理署之「食品原料整合查詢平臺」所載雞母珠之使用部位（根）不符。為釐清茶葉產品中雞母珠之使用部位，本研究應用液相層析飛行式質譜儀（LC-QTOF）篩選出6個雞母珠小葉與7個雞母珠根之特異性成分訊號，並挑選雞母珠小葉特徵成分Hispidulin與Cirsimaritin及雞母珠根特徵成分7-Hydroxy-6-methoxydihydroflavonol作為檢驗標的，建立液相層析串聯式質譜儀（LCMS/MS）之鑑別方法。LC-MS/MS鑑別方法之專一性良好，可應用於茶葉中摻加雞母珠時，釐清其使用部位之檢驗；方法偵測極限於雞母珠小葉特徵成分Hispidulin與Cirsimaritin均為1μg/g，於雞母珠根特徵成分7-Hydroxy-6-methoxydihydroflavonol則為0.5μg/g。本研究後續以市售20件茶葉檢體驗證所建立之檢驗方法適用性，將檢體分別以LC-QTOF與LC-MS/MS方法進行分析，兩者之檢驗結果一致，均為5件檢體檢出雞母珠小葉特徵成分Hispidulin與Cirsimaritin，且所有檢體均未檢出雞母珠根特徵成分7-Hydroxy-6-methoxydihydroflavonol。

The dried leaflets of Abrus precatorius L. commonly known as rosary pea are often used as herbs or tea leaves. However, they have not been included in the“Food Material Integration Query Platform”, which currently lists the roots of A. precatorius. To discriminate which parts of A. precatorius are present in tea leaves, 6 and 7 specific compounds were screened out from the leaflets and roots of A. precatorius by a liquid chromatography-time-of-flight mass spectrometer (LC-QTOF). Specific compounds, including hispidulin and cirsimaritin from the leaflets and 7-hydroxy-6-methoxydihydroflavonol from the root were selected as targets to establish an identification method using a liquid chromatograph/tandem mass spectrometer (LC-MS/MS). The LC-MS/MS method demonstrated good specificity for the analysis of A. precatorius in the tea leaf matrix, with the detection limits of 1μg/g for hispidulin and cirsimaritin from the leaflets, and 0.5μg/g for 7-hydroxy-6-methoxydihydroflavonol from the root. Conducting product analysis on 20 samples, the all 6 specific compounds from leaflets were detected in 5 samples by the LCQTOF method. Additionally, hispidulin and cirsimaritin were also detected in the same 5 samples by the LC-MS/MS method. However, 7-Hydroxy-6-methoxydihydroflavonol was not detected in any sample by either methods.