紅紫檀試管繁殖研究，一種印度Andhra Pradesh地區瀕臨絕種的植物

Propagation Studies of Red Sanders (Pterocarpus santalinus L.f.) In Vitro－An Endangered Taxon of Andhra Pradesh, India

紅紫檀生長於範圍狹小的特定地區，並且是一種不能使用一般方法繁殖的植物。本研究已建立使用枝梢部份繁殖的有效方法，並且依不同層次篩選出影響其快速繁殖的因子。使莖頂芽能夠快速繁殖（再生頻率10-15%）的方法就是把中胚軸培養於B5培養劑中，額外加入3mg/L BAP及1 mg/L的NAA培養六星期。再生莖其莖頂壞死現象已被控制。莖部先處理1 mg/L的IAA，NAA，IBA然後再移入生根培養液中的莖，比事先未處理者能夠長出較好的根。

Red sanders, the pride of Andhra Pradesh, which is confined to a narrow restricted area was selected for the present study based on its major limitations for conventional methods of propagation. Efficient protocols have been established for shoot multiplication in vitro. Factors responsible for rapid multiplication were screened at various levels. The highest shoot bud regeneration frequency (10-15%) was achieved by culturing mesocotyl explants on B5 medium fortified with 3 mg/L 6-benzyl aminopurine (BAP) + 1 mg/L napthalene acetic acid (NAA) within a six week culture period. Shoot tip necrosis expressed in regenerated shoots, was controlled. Shoots treated with indole acetic acid (IAA), NAA and indole butyric acid (IBA) 1 mg/L each prior to transferring them to the rooting medium exhibited better rooting than those with no prior treatment.