玉米基因型在接種病菌或熱休克處理情況下57KDa蛋白的分佈

Destiny of a 57 Kilodalton Protein in Maize Genotypes Responding to Heat Shock and Pathogenic Infections

為了探討玉米幼苗在逆境下，一個57KDa蛋白（Ribosome-associated-protein，RAP）如何由葉綠體轉移至細胞質多核醣體上的機制，乃利用對於Bipolaris maydis感病和抗病的品種為材料，評估這些幼苗接種病菌及熱休克處理情況下的反應。利用Western blot分析的結果證明經由接種或熱休克處理後所產生的RAP是一樣的，而且發現當抗病型的幼苗接菌種之後，此57KDa RAP不會和多醣體接合，它可能繼續停留在葉綠體中。57KDa轉移的現象不僅發生在玉米幼苗中，大豆感染了Phytophthora megasperma後亦有同樣的現象，而且大豆的57KDa RAP和玉米的蛋白可能極為相似。

In an effort to elucidate the mechanism of redistribution of a 57 KDa protein from chloroplasts to cytoplasmic polysomes when maize seedlings encountered a stress, maize genotypes resistant or susceptible to Bipolaris maydis were used for evaluation under inoculated or heat-shocked conditions. Western blot analysis indicated that the redistributed 57 KDa protein caused by a treatment with pathogens or heat shock was identical. When maize seedlings were resistant to pathogens, little amount of this protein could be detected in the cytoplasmic polysome fraction; presumably, the 57 KDa protein remained localized in chloroplasts. Association of this protein with cytoplasmic polysomes in response to stresses is not unique to maize. A preliminary study employing a compatible Phytophthora megasperma-soybean interaction system indicated that soybean also responded to pathogenic stress in that the 57 KDa protein was detected in the preparation of cytoplasmic polysomes.