培養菸草細胞中酸性磷解酶的鑑定及細胞定位

Characterization and Localization of Acid Phosphatases in the Suspension-Cultured Cells of Nicotiana otophora L.

自菸草懸浮培養中收集之細胞，發現其酸性磷解酶大部分位於液泡及細胞壁。菸草葉片及其癒合組織經由聚丙烯醯胺梯度電泳分析，發現其酸性磷解酶之同功酵素圖譜類似。而且菸草懸浮培養之細胞中之酸性磷解酶以管柱色層析法可分出多種酸性磷解酶。它們具有相似之基質特異性，然而鋅離子對它們却表現不同程度之抑制。

Active cells were havested from the tobacco cell suspension culture. The acid phosphatase of tobacco cells was mostly located in the cell wall and vacuole. Similar zymogram of acid phosphatase revealed in the gradient polyacrylamide gel was observed between the tobacco leaves and their induced-callus. Multiple acid phosphatases of tobacco suspension cells separated by chromatography showed similar substrate specificties to most compounds tested. Zn+2 has differential inhibition on the multiple forms of acid phosphatase of tobacco cells.