Analysis of EGFET Based on Static Measurements and Microfluidic System for the RuO2 Lactic Acid Biosensor

Analysis of EGFET Based on Static Measurements and Microfluidic System for the RuO2 Lactic Acid Biosensor

In this study, a potential lactic acid biosensor based on ruthenium dioxide (RuO2) film was proposed. This sensor was measured by a static voltage-time measurement system and dynamic microfluidics system. The sensitivity characteristics of the RuO2 lactic acid (LA) biosensor under different flow rates were studied by dynamic measurement. The sensitivity characteristics were analyzed under the slow flow rates and the fast flow rates. It was found that RuO2 LA biosensor had the better sensitivity (49.80 mV/mM) and linearity (0.996) at the flow rate of 30 μL/min. In this work, the sensitivity and linearity of RuO2 LA biosensor at the optimal flow rate were analyzed. The addition of γ-3-aminopropyltriethoxysilane (APTES) and glutaraldehyde can not only increase the adsorption and functionalization of the enzyme, but also maintain the activity of enzyme. The sensing characteristics were improved in static and dynamic measurements. This sensor has the better sensitivity, linearity, and response time.