| 中文摘要 |
本試驗採用96微米與 5 微米粒徑的馬齒莧（Portulaca oleracea L.）之溶液，依不同濃度分別 ，依不同濃度分別 ，依不同濃度分別 ，依不同濃度分別 作用於巨噬細胞株（ 作用於巨噬細胞株（ 作用於巨噬細胞株（ mouse BALB/c macrophage RAW 264.7）進行抗發炎活性之檢測， ）進行抗發炎活性之檢測， ）進行抗發炎活性之檢測， 包括一氧化氮（nitric oxide, NO）、白介素 6（interleukin 6, IL-6）及腫瘤壞死因子-α（tumor necrosis factor-alpha, TNF-α）等三種細胞發炎激素，以及細胞毒性試驗（microculture tetrazolium assay, MTT assay）。結果顯示，96微米粒徑的馬齒莧，在5.0% 及10.0 % 之測試濃度下，均可顯著抑制 IL-6與TNF-α的產生，而NO則無抑制效果。5微米粒徑的馬齒莧，在0.25%、0.5% 及1.0 %之測試濃度下，皆可顯著抑制 IL-6與TNF-α的產生，而在NO則無抑制效果。同時96 及 5微米粒徑的馬齒莧兩者在MTT細胞毒性檢測方面，皆無顯著之細胞毒害作用。結論，使用96 微米與 5 微米粒徑的馬齒莧溶液，具顯著抑制 IL-6與TNF-α生成之抗炎效果，且未具細胞毒性。此外，粒徑較小之5微米馬齒莧溶液，除有顯著抗炎效果外，更具節省材料與成本之潛能效益，提供馬齒莧進一步的研究與開發應用。 |
| 英文摘要 |
The purpose of this study was to evaluate the effects of 96-micron and 5-micron Portulaca oleracea L. on anti-inflammatory activity of cells. Three pro-inflammatory factors were detected on macrophage cell line (mouse BALB/c macrophage RAW 264.7), including nitric oxide (NO), interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α), and the cytotoxicity (microculture tetrazolium assay, MTT assay) was analyzed. The results showed that 5.0 %, 10% concentration of 96-micron Portulaca oleracea L. solutions administration on cell could inhibit the production of IL-6 and TNF-α, but NO were not affected. The 0.25%, 0.5%, 1.0% concentration of 5-micron Portulaca oleracea L. solutions administration on cell could inhibit the production of IL-6 and TNF-α, but NO were not affected. MTT assay showed that the solutions of 96-micron and 5-micron Portulaca oleracea L. administration had no significant effects on the survival rate of macrophages. The conclusion was that the 96-micron and 5-micron solutions of Portulaca oleracea L. could significantly inhibit the inflammatory effects of IL-6 and TNF-α, and no cytotoxicity. The 5-micron Portulaca oleracea L. solution not only has significant anti-inflammatory effect, but also saves materials and costs. The results could be provided a more in-depth basis for the research of Portulaca oleracea L. |
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