英文摘要 |
Pyricularia oryzae is a filamentous phytopathogen of rice fields worldwide, causing considerable losses to rice production. In the present study, 28 field isolates of rice blast pathogen representing all regions of paddies in Taiwan were assessed for their taxon, mating type, and fungicide sensitivity. All isolates were identified as P. oryzae and significantly different from P. grisea based on the concatenate sequences of internal transcribed spacer, actin, and calmodulin genes. Only Mat1-1 mating type was present among all test isolates based on polymerase chain reaction assays. According to microtiter plate assays, mancozeb and acidified calcium propionate, potassium sorbate, and sodium benzoate each could completely inhibit conidial germination and mycelial growth of all isolates. In addition, epoxiconazole followed by iprobenfos and probenazole exhibited a good inhibition effect on their spore germination. On the other hand, epoxiconazole and prochloraz could inhibit mycelial growth of all isolates, and carbendazim, iminoctadine tris (albesilate), and tebuconazole inhibit the growth of most isolates. Besides, fenoxanil and tricyclazole inhibited appressorium formation showing great potential in controlling the rice blast. |