菜豆普通嵌紋病毒（BCMV）與胡瓜嵌紋病毒（CMV）的萊豆（Phaseolus limensis）分離株之鑑定與發生

Occurrence and Identification of Bean Common Mosaic Virus and Cucumber Mosaic Virus Isolated from Lima Bean (Phaseolus limensis)

萊豆（Lima bean）一般俗稱皇帝豆，藥用時稱為觀音豆，台灣以蔓性白仁大粒種（Phaseolus limensis Macf.）為主要栽培品種。萊豆係自交作物，農民習慣自行留種，因此有種子帶病毒情形發生。台灣萊豆產期在1-3月，秋冬時期與多種豆類作物同時生長，易導致豆類的病蟲害相互傳染，尤以萊豆植株生長勢強而枝葉密集，病蟲藏匿其中，作物健康管理無法徹底執行。

"Two virus isolates, BCMV-c9 and CMV-Lima, were isolated from diseased lima bean plants (Phaseolus limensis Macf.) cultivated in Taichung and Tainan. After local-lesion isolation on Chenopodium quinoa, BCMV-c9 was identified as a Bean common mosaic virus (BCMV) by ELISA and CMV-Lima was a Cucumber mosaic virus (CMV) tested by SDSimmunodiffusion. For further identification, potyvirus specific primer (Pot1/HRP5) and cucumovirus specific primer (CPTALL-3/CPTALL-5) were adopted for reverse transcription polymerase chain reaction (RT-PCR). By nucleotide sequence alignment, the CP gene of BCMV-c9 was 89.2% identical to that of Accession number U34972, a BCMV-PSt strain. Phylogenetic analysis also showed that BCMV-c9 was closer to BCMV-PSt strains than to BCMV-BlCM strains. CMV-Lima shared more than 95% of identities with CMV isolates Tfn and NT9 which belong to IB subgroup, 93-94% with isolates Fny and Y which belong to IA subgroup, and only 77-78% with isolates Q and LS which belong to IA subgroup. Consequently, BCMV-c9 is identified as a BCMV-PSt strain and CMV-Lima is a CMV strain that belongs to subgroup IB. From the field survey showed that BCMV was widespread in lima bean cultivated areas, and CMV was occasionally occurred."