Analysis of heterocyclic aromatic amines using selective extraction by magnetic molecularly imprinted polymers coupled with liquid chromatography-Mass spectrometry   全文下載

Analysis of heterocyclic aromatic amines using selective extraction by magnetic molecularly imprinted polymers coupled with liquid chromatography-Mass spectrometry

"Heterocyclic aromatic amines (HCAs) are highly carcinogenic and mutagenic chemicals. This study reports on thedevelopment of magnetic molecularly imprinted polymers (MMIPs) for the purification and quantification of HCAs. Anovel magnetic molecularly imprinted polymer was successfully prepared using a surface molecular imprinting methodusing functionalized Fe particles as the magnetic cores. 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) was used as amolecular template; methacrylic acid (MAA), ethylene glycol dimethyl acrylate (EGDMA), 2, 2'-Azobis (2-methylpropionitrile)were used as the functional monomer, crosslinker, and initiator, respectively. The use of the template/functional monomer/crosslinking agent at a ratio of 1:4:20 resulted in a product with better adsorption properties(3.24 mg/g). The HCAs were successfully detected and quantified in processed meat samples by MISPE and LC-MS/MS.Under the final optimized detection conditions, the proposed method offered good linearity (R > 0.995) for the five HCAswith an acceptable level of precision, and an LOQ of 0.05 ng/g was successfully achieved."