印度餘甘子提取物抑制發炎作用之研究

Study on the Anti-inflammatory Effect of Indian Phyllanthus Emblica Extract

"在傳統醫學應用中，藥用食材植物中的活性成分，一直是提供傳統臨床療法的重要來源之一。本文探討印度餘甘子（Phyllanthus emblica L.）對於生物體發炎的緩解或消除之抗發炎作用，並對其傳統醫學應用之安全性研究加以彙整。吾等以印度餘甘子提取物為素料，分析在培養的動物細胞株中，對於發炎反應相關的三種因子，觀察其是否具有抑制作用。所分析的三種因子包括了一氧化氮（nitricoxide,NO）、介白素6（interleukin6,IL-6）、腫瘤壞死因子-α（tumornecrosisfactor-alpha, TNF-α），同時也進行細胞毒性（microculture tetrazolium assay, MTT assay）分析。結果顯示，印度餘甘子水萃取液對於受到細菌脂多醣（lipopolysaccharide, LPS）活化的巨噬細胞株，具顯著抗發炎反應，可抑制LPS所誘發NO與IL-6之生成，對於NO之抑制率為36.0%，對IL-6之抑制率為31.4%，而對TNF-α生成則無顯著作用。此外，在上述測試濃度之下，皆未測得細胞毒性。綜上所述，印度餘甘子提取物被使用於傳統經驗及細胞層面之研究，皆呈正面功效。即便如此，對於餘甘子的整體臨床治療效果，仍需要更多高質量的相關研究，為健康管理的應用提供支持。"

In traditional medicine applications, the active ingredients in medicinal food plants have always been one of the important sources for providing traditional clinical treatments. In this study, we investigated the anti-inflammatory effects of Indian Phyllanthus emblica L. on the alleviation or elimination of inflammation in organisms and summarized the safety data of Phyllanthus emblica L. in the application of traditional medicine. We analyzed three factors related to the inflammatory response in cultured animal cell lines using the water extract of Indian Phyllanthus emblica L. as research material to see if it had inhibitory effects. These three factors we analyzed included nitric oxide (NO), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-α). At the same time, the cytotoxicity analysis of microculture tetrazolium assay (MTT assay) was also performed. The results show that the extract of Phyllanthus emblica L., for macrophage cell strains activated by bacterial lipopolysaccharide (LPS), has a significant anti-inflammatory response. It inhibited the production of NO and IL-6 induced by LPS. The inhibitory rates of NO and IL-6 were 36.0% and 31.4% respectively, but there was no significant effect on the production of TNF-α. Besides, no cytotoxicitywas detected at the above test concentrations. Based on the above description, the results of both traditional experience and cellular studies show positive effects. Even so, for the overall clinical treatment effect of Phyllanthus emblica L., it still needs more high-quality related research to support the application of health management.