中藥『三黃散』對燙傷的療效研究

Efficacy of Traditional Chinese Medicine“San Huang Powder”in Burn Wound Healing in a Porcine Model

目的：燒燙傷的中醫外用製劑，常使用大黃、黃連、黃檗等清熱解毒藥，來治療燒燙傷後的傷口。本實驗以治療燒燙傷的西藥『燙膚舒』為對照組，比較大黃、黃連、黃檗組成的『三黃散』對淺二度燙傷的治療效果。方法：由於豬隻的體型大小與皮膚結構，皆與人類最為接近，故選擇豬隻為實驗動物。傷口癒合的過程中，在第7、14、21天以照相記錄傷口外觀，同時將組織切片以蘇木精－伊紅染色（Hematoxylin and Eosin stain；以下簡稱H&E）、馬森三色染色（Masson's trichrome stain；以下簡稱MT）和免疫組織化學染色（Immunohistochemistry；以下簡稱IHC），比較『三黃散』與燙膚舒對燙傷傷口癒合的差異。結果：在傷口癒合過程的組織病理學評估，三黃散對於皮下組織的纖維母細胞增殖和膠原蛋白合成、血管新生的表現皆優於燙膚舒對照組。動物實驗的結果顯示，沒有任何實驗動物出現感染症狀。結論：本研究結果顯示了『三黃散』對淺二度燙傷療效，並對將來改進傳統中藥製劑『三黃散』提供了基礎。

Objectives: ''San Huang Powder'' is frequently used for the treatment of burn wounds in traditional Chinese medicine. It was prepared from the following three yellow-colored herbs: Rhubarb, Phellodendron bark, and Coptidis rhizome in this study. Methods: Because the pig's size and skin composition are the closest to the human body, the pig was employed as the experimental animal. Western medicine, Siliverzine cream, was used as the control. Histopathological evaluation, including MT staining, H&E staining, and IHC staining, of dermal tissue healing, was carried out to compare the efficacy of ''San Huang Powder'' and the control after surgery on day 7, 14, and 21. Results: In the histopathological evaluation of the wound healing process, San Huang Powder is better than Siliverzine cream in terms of fibroblast proliferation, collagen synthesis and angiogenesis in the subcutaneous tissue. The results of animal experiments showed that no wound infection was observed in all experimental animals. Conclusions: The results of this study show the efficacy of ''San Huang Powder'' on superficial second-degree scalds and provide the basis to improve the preparation of this traditional medicine.