| 英文摘要 |
Antlers are non-ossified baby horns, which can enhance physical health, improve immunity, and exhibit rare mammalianregenerative abilities. In Chinese culture, antlers are considered a valuable herbal medicine. Formosan Sambar deer arecommonly used for antler collection and it has already been shown that Sika deer antler stem cells have a distinct set of usefulproperties, but Formosan Sambar deer antlers stem cells have not been extensively studied. This study isolated Formosan Sambardeer antler stem cells (DASCs), and conducted cell identification by RT-PCR. Differentiation of the mesoderm led to successfulpassaging of chondrocytes, osteoblasts, and adipocytes. Results showed that the DASCs were successfully isolated and expressedin CD29, CD105, and CD45. Tri-lineage differentiation potential was confirmed through staining with Oil Red O, Toluidine BlueO, and Alizarin Red S, respectively. Following convention, CD29 and CD105 were taken as the stem cell-relative markers. CD45was also found, and this is related to angiogenesis; thus it may explain some of the regenerative tissue properties associated withantlers. DASCs a beginning to show great potential as a source of experimental cells, with potential downstream applications inthe fields of health care and regenerative medicine. |
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