Due to professional drivers' long working hours on the road, drivers' careless mistake often leads to a traffic accident that not only endangers the drivers' personal life but also puts passengers and other roadway users in a potential hazardous situation. In recent years, the increase in traffic density further worsens the drivers' working environment hence adding varying degrees of job pressure to the workers. If the driver cannot adjust to the pressure, it will affect the drivers' personal health, the organization's performance and passengers' well being. Since the external environment cannot be controlled, the issue of how to assist the driver to better adjust and face a variety of working conditions and job stress is one of the most important issues to bus operators. This research was focused on emotional intelligence to study its effect on the relationship between work stressor and stress reaction. It can help organizations train up their staff's emotional intelligence in reducing damage caused by work stress to workers and organizations. This will improve the organizational performance. The results showed a striking effect of the work stressor and the five dimensions of work stressor on stress reactions. The empirical study indicated that there is a negative effect on job satisfaction and a positive effect on negative stress reaction. Specifically, the effect on job satisfaction from the stressor of work itself is more than its effect on negative stress reaction, in the five dimensions of work stressor. However, the five dimensions of work stressor do not affect the stress reactions at the same time. Only organization structure do significantly and negatively influences job satisfaction. Work itself has a significantly positive effect on the negative stress reaction. Finally, we discussed the managerial implications and proposed some suggestions to future research.