化粧品中色素成分分析方法之建立   全文下載

Establishment of Analytical Method for Banned and Restricted Dyes in Cosmetics

本研究係利用液相層析串聯質譜儀（Liquid Chromatograph／Tandem Mass Spectrometer, LC-MS／MS）建立一簡單、快速的方法，可同時分析18種限用化粧品色素成分；利用正負離子電灑離子化法（ESI），搭配多重反應偵測模式（multiple reaction monitoring, MRM）偵測，可在16分鐘內完成分析。以唇蜜為空白檢體，添加適量標準品原液，使最終濃度為0.2－400ppm（依檢測品項之感度選定添加濃度），進行連續三天及五重複之添加回收試驗，其平均回收率為99.1－109.3％，變異係數均小於6.89％。結果證實本研究所建立之LC／MS／MS方法可以快速分析及定量化粧品中18種色素。

A simple and fast method was developed for simultaneous determination of 18 dyes in cosmetics by liquid chromatography/tandem mass spectrometry (LC-MS/MS). The analysis could be accomplished within 16 min by employing electrospray ionization (ESI) with the multiple reaction monitoring (MRM) mode for detection. The recoveries were assayed to be form 99.1% to 109.3%, by spiking multiple concentrations levels of standards ranging between 0.2 and 400 ppm in lip gloss samples. Intraday and interday precisions (relative standard deviations) were all less than 6.89%. The results validated that the LC-MS/MS method was a fast and quantitative technique readily used for simultaneous determination of dyes in cosmetics.