Aim: Mycoplasma pneumoniae is one of the most common pathogens for respiratory tract infections in children and adults. Macrolide is the drug of first choice for the treatment of children with Mycoplasma pneumonia infection. However, more and more studies in the past 10 years have found that mycoplasma infection can cause severe pulmonary infections and even deaths. In the present study, we were aimed to investigate molecular characters of culture proven Mycoplasma pneumoniae isolates from a regional hospital in central Taiwan.
Methods: We conducted a cross section study, which enrolled clinical diagnosed MP infected children of ages less than 14-years-old. In addition to serum mycoplasma IgM tests, we exploited polymerase chain reaction and SP4 culture to confirm MP infection. For further investigation, we only characterized culture proven MP isolates by using Multiple-Locus Variable number tandem repeat Analysis (MLVA) and P1 gene typing, and 23S rRNA (domain V) gene sequencing to study genotype distribution and Macrolide resistance rate.
Results: In total, 76 clinical samples were collected and 29 out of them were culture positive. Four different MLVA types were found in 29 positive strains, including J, P, A and X types. Two prevalent MLVA patterns dominated in this study, which were 4-5-7-2 and 4-5-7-3.The P1 gene type distribution accounted for 28 (96.6%) of P1-1 and only 1 (3.4%) of P1-2. Five of the 29 culture positive strains (5/29;17.2%) revealed macrolide resistant mutations (A2063G).
Conclusion: This study showed that MLVA 4-5-7-2 and 4-5-7-3 type MP prevailed in the community in Central Taiwan. The macrolide resistant rate was 17.2%, which is comparable to previous studies.